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. 2013 Nov 20;8(11):e79284. doi: 10.1371/journal.pone.0079284

Figure 3. Methotrexate and/or BV effect on Tissue necrosis factor-alpha expression in synovial membrane of hind paw.

Figure 3

Immunohistochemical staining (×100) of paw sections of A: normal rat shows almost negative immunostaining for TNF-α, B: arthritic non-treated rat shows massive immunostaining, C: MTX treated rat shows moderate immunostaining, D: BV treated rat shows mild immunostaining and E: concurrently treated rat with MTX and BV shows minimal TNF-α expression. F: mean optical density of synovial membrane stained with TNF-α immunostaining in different studied groups. Data are represented as mean ± SD (n = 10). a, b, c or d: significantly different from the corresponding Normal, Arth, MTX or BV group respectively at P<0.05 using one-way ANOVA followed by Tukey-Kramer Multiple comparison test.