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. 2013 Nov 20;8(11):e72411. doi: 10.1371/journal.pone.0072411

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© 2013 Nie et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Flow cytometry analysis of Th1, Th2, Th17 and Treg cell populations in spleen. (B) Summarized data of Th1, Th2, Th17 and Treg cell populations in spleen. (C) mRNA expression of T-bet, GATA3, RORγ and Foxp3 in splenic lymphocytes were detected by qPCR. (D) Inhibitory function of Treg cells in each group were detected by mixed lymphocyte reaction. (E) The mean mass of spleen for each group (Mean ± s.e.m.). *P<0.05, **P<0.01, ***P<0.001, or ns = No significant difference.