Table 4. Kinetic parameters of PcGSTFuAs and CcGSTFuAs towards MUA in absence or presence of GSH.
| Km (μM) | PcGSTFUA1 | PcGSTFUA2 | PcGSTFUA3 | PcGSTFUA4 |
| MUA (−GSH) | 262.8±33.7 | 23.5±6.2 | 33.89±4.78 | 51.56±7.09 |
| MUA (+GSH) | 206.7±23.3 | 216.1±45.2 | 372.1±53.9 | N.D |
| kcat (min−1) | PcGSTFUA1 | PcGSTFUA2 | PcGSTFUA3 | PcGSTFUA4 |
| MUA (−GSH) | 0.095±0.004 | 0.302±0.006 | 0.182±0.003 | 0.208±0.004 |
| MUA (+GSH) | 57.83±2.90 | 1.27±0.14 | 29.65±2.36 | N.D |
| kcat/Km (min−1/um−1) | PcGSTFUA1 | PcGSTFUA2 | PcGSTFUA3 | PcGSTFUA4 |
| MUA (−GSH) | 0.36*10−3±0.12*10−3 | 0.0128±0.0001 | 5.37*10−3±0.62*10−3 | 4.03*10−3±0.56*10−3 |
| MUA (+GSH) | 0.28±0.12 | 5.87 10−3±3.1 10−3 | 0.079±0.044 | N.D |
| Km (μM) | CcGSTFuA2461 | CcGSTFuA6800 | CcGSTFuA6801 | CcGTSFuA6820 |
| MUA (−GSH) | 38.7±7.1 | 25.39±4.37 | 182.3±29.4 | 16.9±2.1 |
| MUA (+GSH) | N.D | 111.7±11.1 | 189.7±7.3 | N.D |
| kcat (min−1) | CcGSTFuA2461 | CcGSTFuA6800 | CcGSTFuA6801 | CcGTSFuA6820 |
| MUA (−GSH) | 0.026±0.001 | 0.065±0.002 | 0.070±0.004 | 0.078±0.002 |
| MUA (+GSH) | N.D | 5.10±0.18 | 7.79±0.13 | N.D |
| kca/Km (min−1/um−1) | CcGSTFuA2461 | CcGSTFuA6800 | CcGSTFuA6801 | CcGTSFuA6820 |
| MUA (−GSH) | 0.67*10−3±0.14*10−3 | 2.56*10−3±0.46*10−3 | 0.38*10−3±0.13*10−3 | 4.61*10−3±0.9*10−3 |
| MUA (+GSH) | N.D | 0.046±0.016 | 0.041±0.017 | N.D |
The apparent Km values were determined using a concentration range of 100–800 µM in absence (−GSH) or presence of 5 mM GSH (+ GSH). The apparent Km and kcat values were calculated by nonlinear regression using the Michaelis-Menten equation (r2>0.99). Data are represented as mean ± S.D. (n±3). ND: Not Detected. The detection limit was estimated at 0.5 mUI.