Table 5. Competition experiments between ANS or MUA and wood compounds.
| PcGSTFuA1 | PcGSTFuA2 | PcGSTFuA3 | PcGSTFuA4 | |
| Fluorescent probe | ANS | ANS | ANS | ANS |
| Coniferaldehyde | 62.3±1.2 µM | 115.6±44.1 µM | 68.4±25.3 µM | 146.6±5.1 µM |
| Vanillin | 217.8±8.5 µM | 84.7±5.2 µM | 134.0±7.6 µM | 308.9±29.3 µM |
| Methoxybenzophenone | - | - | - | - |
| 4-chloro-3-nitrobenzoic acid | 1.5±0.1 mM | 1.3±0.2 mM | 234.7±56.7 µM | - |
| 4'-hydroxyacetophenone | 2.2±0.4 mM | - | - | - |
| Gallic acid | - | - | - | - |
| Vanillic acid | - | - | - | - |
| Epicatechin | 1.6±0.1 mM | - | - | - |
| Syringaldehyde | 62.5±1.6 µM | 89.3±2.8 µM | 37.2±3.8 µM | 198.5±25.2 µM |
| Catechin hydrate | 575.3±98.7 µM | - | - | - |
| Metoxycinnamic | - | - | - | - |
| CcGSTFuA2461 | CcGSTFuA6800 | CcGSTFu6801 | CcGSTFuA6820 | |
| Fluorescent probe | MUA | ANS/MUA | ANS | MUA |
| Coniferaldehyde | - | ND | 327.4±50.6 µM | 768.8±98.4 µM (non-competitive) |
| Vanillin | - | ND | 423.6±107.3 µM | - |
| Methoxybenzophenone | - | ND | - | - |
| 4-chloro-3-nitrobenzoic acid | 287.4±29.7 µM (competitive) | ND | 2.3±1.3 mM | 1.3±0.03 mM (non-competitive) |
| 4'-hydroxyacetophenone | 352.1±25.1 µM (competitive) | ND | 2.9±1 mM | 1.2±0.03 mM (non-competitive) |
| Gallic acid | 113.9±8.9 µM (competitive) | ND | - | 566.4±16.5 µM (non-competitive) |
| Vanillic acid | 1±0.1 mM (competitive) | ND | - | - |
| Epicatechin | - | ND | 3.9±2.3 mM | 1.4±0.04 mM (non-competitive) |
| Syringaldehyde | - | ND | 275.8±97.8 µM | 594.2±37.6 µM (non-competitive) |
| Catechin hydrate | - | ND | 3.7±1.7 mM | 564.2±12 µM (non-compet) |
| etoxycinnamic | - | ND | - | 1.5±0.04 mM (non-competitive) |
For ANS competition, the values given are IC50 obtained by fitting data to equation 2 described in Material and Methods.
For MUA competition, the values given are Ki obtained by fitting data to noncompetitive or competitive inhibition of esterase activity in the presence of 0 to 10 mM inhibitor.
The apparent Ki values were calculated by nonlinear regression using the competitive or noncompetitive inhibitions equations described in Material and Methods (r2>0.99). Data are represented as mean ± S.D. (n±3). ND: Not determined since no competition was observed with GSH for ANS and MUA.