Figure 1. Structures of CesAB, CesAB–EspA and CesABs.
(A) Solution structure of the homodimeric CesAB, which adopts a molten-globule-like structure in solution (Chen et al., 2011).
(B) 1H-15N HSQC spectrum of CesAB.
(C) Crystal structure of the heterodimeric CesAB–EspA (Yip et al., 2005). Regions of the proteins that were not crystallographically resolved are represented as dotted lines.
(D) 1H-15N HSQC spectrum of CesAB–EspA. The CesAB subunit is 15N labeled whereas the EspA subunit is unlabeled.
(E) Solution structure of the CesABs variant (D14L/R18D/E20L), determined in this work.
(F) 1H-15N HSQC spectrum of CesABs
(G) Superposition of the CesAB subunit of CesAB, CesAB–EspA and CesABs.