A, garcinol dose dependently inhibited the invasion of MDA-MB-231 cells. B and C,
effect of NF-κB p65 and anti-miR-200 transfection on garcinol-induced
inhibition of invasion of MDA-MB-231 cells. C, control (vehicle treatment); G, cells
treated with 25 μmol/L garcinol; G + p65, cells transfected with
NF-κB p65 followed by 25 μmol/L garcinol treatment, G +
miR-200s, cells transfected with anti-miR-200s (anti-miR-200a +
anti-miR-200b + anti-miR-200c) followed by garcinol treatment. Bar graphs
represent quantitation of the degree of invasiveness as determined by fluorescence
values under each condition. *, P < 0.05 and
**, P < 0.01 versus vehicle control. AFU,
arbitrary fluorescence units.