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Intracellular staining for TSH-βv. BMDMØs were cultured from fresh BM for 7 days with MCSF (10 ng/mL) and their nuclei labeled with 4′,6′-diamidino-2-phenylindole (DAPI) (A) and stained for F480+ surface antigen (B) and intracellular TSH-βv with anti-TSH (C). The overlay (D) illustrates that MØs contain intracellular TSH-βv (×630).
