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. Author manuscript; available in PMC: 2014 May 15.
Published in final edited form as: Science. 2013 Nov 7;342(6160):10.1126/science.1240585. doi: 10.1126/science.1240585

Fig.4. Met-tRNAiMet conformation on the initiation complex.

Fig.4

(A) Cryo-EM density for the Met-tRNAiMet allowed unambiguous docking of the tRNA (green) and the start codon of mRNA (purple). (B) Met-tRNAiMet (green) in the initiation complex compared to canonical P-site tRNA conformation (orange, PDB ID 2WDK/2WDL)(17). A displacement in the anticodon stem loop (ASL) is indicated. The 3′ CCA of the Met-tRNAiMet is stabilized in a conformation that prevents its entering the peptidyl transferase center (PTC), circled.