Fig. 2.

(A) Sequence of head domain of neuraminidase (HNA). The gray color shows the sequence of the HNA. The box indicates FLAG tag. (B) Plasmid constructed for yeast surface display of the HNA. Head, head domain. (B) A microscopic observation of surface display after immunofluorescence staining. Forty-eight hours after cultivation of the yeast, the display of NAs on yeast was examined by immunofluorescence staining using an anti-FLAG M2 mouse IgG antibody followed by an Alexa Flour 488-conjugated anti-mouse IgG antibody. The yeast suspension was transferred onto a glass slide and observed under a fluorescence microscope. Scale bars indicate 5 μm.