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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: J Immunol. 2013 Jul 22;191(4):10.4049/jimmunol.1300284. doi: 10.4049/jimmunol.1300284

Figure 5. Allergens from three different structural classes sensitize signalling by TLR4 and TLR2.

Figure 5

(A) Wild-type and TLR4−/− BMDM treated with LPS at 0.25ng/ml in the presence, or absence, of the dog lipocalin allergen Can f 6 at 50μg/ml for 24 hours. TNF-alpha levels were measured by an ELISA (R&D). Data from 3 separate experiments were pooled and expressed as mean±sem. (B) Wild-type and TLR4−/− BMDMs treated with LPS (0.25ng/ml), the house dust mite allergen Der p 2 (25μg/ml), the model allergen protein ovalbumin (OVA, 20μg/ml) or cat derived Fel d 1 (NFel d 1, 25μg/ml) either singly or in combination for 24h. TNF-alpha levels were measured by an ELISA (R&D). Data from 3 separate experiments were pooled and expressed as mean±sem. *P<0.05; **P<0.005;***P<0.001; n.s., not significant.