Skip to main content
. Author manuscript; available in PMC: 2013 Nov 21.
Published in final edited form as: Oncogene. 2008 Mar 10;27(29):10.1038/onc.2008.49. doi: 10.1038/onc.2008.49

Figure 1. Effects of WT and Mutant PTEN Genes on Colony Formation and Proliferation.

Figure 1

Panel A) MCF-7 cells were transfected with plasmid DNA encoding PTEN(WT), PTEN(C124S), PTEN(G129E), PTEN(399stop) or the empty vector pEGFP-C2. The mean number colonies present after selection in 2 mg/ml G418 for approximately 3 weeks and corresponding standard deviation was calculated from 9 replicate wells for each plasmid. Asterisks indicate statistically significant decreases in the number of colonies present in comparison to pEGFP-C2 transfected cells (***, P<0.001). Panels B–E) Proliferation of: MCF/EGFP-C2 cells (open circles, ○) MCF/PTEN(WT) (solid squares, ■), MCF/PTEN(C124S) (solid triangles, ▲), MCF/PTEN(G129E) (solid diamonds, ◆), and MCF/PTEN(399stop) (solid circles, ●) in the presence of 0 nM (Panel B) 125 nM doxorubicin (Panel C), 500 nM doxorubicin (Panel D) or 2,000 nM doxorubicin. The amount of cells present after incubation in these conditions for 1, 2, 3, or 4 days was normalized to the mean amount of cells present at the time of treatment.