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. Author manuscript; available in PMC: 2013 Nov 21.
Published in final edited form as: Oncogene. 2008 Mar 10;27(29):10.1038/onc.2008.49. doi: 10.1038/onc.2008.49

Figure 4. Effects of PTEN Expression on Akt, Ask-1 and p70S6K Phosphorylation.

Figure 4

Cells were collected after trypsinization and plated in RPMI 1640 containing 10% FBS. After allowing the cells to adhere for 24 hrs, the monolayers were washed twice with PBS and then phenol red free RPMI 1640 containing 0.5% CS FBS was added for 24 hrs. Cells were then stimulated with 10% FBS for the indicated time periods. Protein lysates were prepared and subjected to western blot analysis.