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. Author manuscript; available in PMC: 2014 May 17.
Published in final edited form as: ACS Chem Biol. 2013 Mar 29;8(5):10.1021/cb3007314. doi: 10.1021/cb3007314

Figure 4. Overall representation of the dual imaging concept for luciferin ligation.

Figure 4

Both luciferin ligation precursors could be caged as sensors for two different biomolecules. Only when both become uncaged D-luciferin or D-aminoluciferin is formed as the result of split luciferin ligation reaction, allowing the production of light by luciferase.