Figure 2. PI-103 activates caspase-9 and -3 in CEM-R and MOLT-4 cells, while rapamycin activates caspase-9 and -3 in MOLT-4 cells only.
CEM-R and MOLT-4 cells were treated with PI-103 (0.75 µM) or rapamycin (0.1 µM) for the indicated times, collected, and then lysed. Fifty micrograms of each lysate were electrophoresed on SDS-PAGE gels followed by transfer to nitrocellulose membrane.