Fig. 2.

Lactate dehydrogenase (LDH) release versus time in rat (A) and human (B) sandwich-cultured hepatocyte (SCH) experiments performed according to the scheme depicted in Fig. 1A(i) and (iii) during the uptake and efflux phase, respectively. Closed symbols represent SCH treated in standard (+Ca²⁺) Hanks' balanced salt solution (HBSS) with intact bile networks, whereas open symbols represent cells treated with Ca²⁺-free HBSS during the preincubation and efflux phase with open bile networks maintained throughout the study period. Data are reported as mean ± S.E.M. (n = 3 SCH preparations in triplicate).