Fig. 8.

Cell cycle analysis after LY294002 treatment. NCCIT cells were exposed to 0 or 4 Gy γIR, plated, incubated at 37°C for 1 hour before treatment with DMSO vehicle or LY294002 (LY) (6.25 μM). Cells were incubated for 21 or 41 hours and then treated with 10 μM 5-ethynyl-2′-deoxyuridine (EdU). After an additional 6 hours of incubation, the cells were harvested and analyzed with FACSCalibur. (A and B) Representative flow cytometry profiles. The cell cycle phases are outlined, with the upper quadrant indicating the S phase cell population, the lower right being the G₂/M phase population, and the lower left reflecting the G₁ phase cells. Quantitative cell cycle distribution 28 hours (C) or 48 hours (D) after IR exposure. Black columns indicate nonirradiated cells, and white columns indicate irradiated cells. N = 3. Bars = S.E.M. *P < 0.05, Student’s t test.