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. 2013 Nov 6;2013:827905. doi: 10.1155/2013/827905

Figure 2.

Figure 2

Antiproliferative activity and cell cycle arrest activity of PDGF-bb with EGCG on RAOSMC. After 24 h of starvation with serum-free DMEM, the cells were treated with 10 ng/mL PDGF-bb and increasing concentrations (10–80 μM) of EGCG for 24 h. (a) The effects of EGCG growth inhibition on PDGF-bb stimulation in RAOSMC. Cell viability was detected using an MTT assay. *P < 0.05 compared with nonstimulation control; # P < 0.05 compared with the 10 ng/mL PDGF-bb stimulated control. (b) The effects of EGCG on PDGF-bb-induced DNA synthesis in RAOSMC. DNA synthesis was detected using the BrdU incorporation assay. *P < 0.05 compared with nonstimulation control; # P < 0.05 compared with 10 ng/mL PDGF-bb stimulated control. (c) The effect of EGCG on PDGF-bb stimulated cell cycle distribution in RAOSMC. Cell cycle distribution was determined by propidium iodide (PI) labeling followed by flow cytometry. The percentages of cells in the G0/G1, S, and G2/M phases were calculated using Modifit computer software and represented within the histograms.