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. 2013 Jul 30;208(12):2067–2074. doi: 10.1093/infdis/jit331

Figure 6.

Figure 6.

DPA-713 accumulates within macrophages. Lungs from infected C3HeB/FeJ mice (A–E) and infected but translocator protein (TSPO)–auto-blockaded mice (F–J) were harvested and processed 24 hours following intravenous administration of DPA-713-IRDye680LT (fluorescent analog of DPA-713). Signal from DPA-713-IRDye680LT (B and G; red), TSPO (C and H; white), CD68 (D and I; green), overlay of CD68 with DPA-713-IRDye680LT (E and J), and overlay of all channels with nuclear stain (A and F) are shown. DPA-713-IRDye680LT signal is clearly noted in lung tissues obtained from the infected mouse (B; red) and colocalizes with CD68 (E; yellow). However, while both TSPO (H) and CD68 (I) signals are visible in the lung tissues from the infected but TSPO–auto-blockaded mouse, no DPA-713-IRDye680LT signal is noted (G). Arrows point to a cluster of macrophages or CD68+ phagocytic cells. Scale bars represent 25 μm.