Skip to main content
. 2013 Aug 2;208(12):2046–2057. doi: 10.1093/infdis/jit398

Figure 5.

Figure 5.

Incubation of platelets with LTA increases cAMP concentrations. A, Platelets (8 × 108 cells/mL) were pretreated with either Ginkgolide B (2 mM) or tyrodes buffer for 30 minutes. Platelets were then treated with LTA from Staphylococcus aureus SA113 (4 µg/mL), PGI2 (0.25 µg/mL), supernatant from S. aureus SEJ1, SEJ1 ΔgdpP, SEJ1 ΔltaS ΔgdpP or SEJ1 ΔltaS ΔgdpP pCN34-ltaS (10 µg/mL), or Tyrodes buffer. Lysates were immunoblotted with an anti-VASP antibody. B, Platelets (8 × 108 cells/mL) were pretreated with either Ginkgolide B (2 mM) or tyrodes buffer for 30 minutes. Platelets were then treated with LTA (4 µg/mL), PGI2 (0.25 µg/mL), or tyrodes buffer. Samples were then assayed for cAMP concentration determined by ELISA. Data represent mean values ± SEM. *P < .05. Abbreviations: cAMP, cyclic adenosine monophosphate; ELISA, enzyme-linked immunosorbent assay; LTA, lipoteichoic acid; SEM, standard error of the mean; VASP, vasodilator-stimulated phosphoprotein.