Figure 6.

LTA inhibits thrombus formation in vitro. A, Platelets within whole human blood were labelled with a lipophilic dye DIOC6. Whole blood was then treated with (Ai) tyrodes buffer, (Aii) LTA extracted from Staphylococcus aureus strains SA113 (10 µg/mL) or (Aiii) LTA extracted from S. aureus strain SA113 ΔdltABCD (10 µg/mL) for 15 minutes. Whole blood was then perfused through collagen coated (400 µg/mL) Vena8Biochip at a flow rate of 20 dynes cm⁻². Formation of thrombi was recorded using a Z stack capture every 30 seconds for 10 minutes using a Nikon eclipse (TE2000-U) microscope. Thrombus fluorescence intensity was calculated using Slidebook 5 software. B, Data represents mean of thrombi volume over the experiment duration. C, Data represent mean ± SEM of peak fluorescence intensity. *P < .05. **P < .01. Abbreviations: LTA, lipoteichoic acid; SEM, standard error of the mean.