Figure 2.
Methylation profiles of the APOE CGI from human PMB tissues and lymphocytes. (A) The mean percentage of methylation at the 75 targeted CpG sites across tissues (n = 14 or 15) and the SNP rs429358 site for ɛ4/ɛ4 homozygous samples only (denoted by filled diamonds, n = 2 or 3). (B) For each tissue type, methylation percentages from all 15 subjects were averaged at each of the 75 CpG sites. The range and mean (denoted by filled black diamonds) methylation percentages across these 75 sites are displayed in a box-and-whisker plot. Mean methylation proportions across all 75 CpG sites were transformed, and statistically significant differences between all five tissues tested were detected (Holm-adjusted P < 10−10 for all 10 pair-wise comparisons).