Fig. 1.

Predicted RNA secondary structures within the NPHV 5′UTR. (a) The nucleotides corresponding to the 5′ terminus and AUG start codons of HCV are boxed and underlined, respectively. Domains II and III(a–f) of the prototype HCV IRES are labelled. Sequences corresponding to microRNA-122 binding sites within HCV are represented by black lines above the primary sequence. Figure adapted from Burbelo et al. (2012). (b) Schematic of the bicistronic transcripts used to assess the potential IRES function of hepaciviral 5′UTR sequences.