Figure 1.

GLP-1R is expressed in ARPE-19 cells. (a) RT-PCR showing GLP-1R mRNA expression in human ARPE-19 cells cultured in standard medium. The expected RT-PCR product size is 98 bp. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was utilized as an external control. Representative agarose gel of three different experiments. (b). Representative western blot analysis of human GLP-1R protein levels (∼65 kD) showing that GLP-1R protein is expressed in ARPE-19 cells. Lysates from the pancreatic insulin secreting cell line HIT-T15 were used as a positive control. Representative western blot analysis of three different experiments is shown.