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GLP-1 does not affect secretion of VEGF-A. ARPE-19 cells were cultured for 24 h in in the presence or absence of 10 nmol/L GLP-1. The conditioned media were collected, and VEGF-A levels in supernatants were assessed by ELISA. VEGF-A concentration was calculated from standards curve and normalized to total protein concentration of the respective lysates. The results are representative of three independent experiments (mean ± SE).
