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. 2010 Feb 22;3(2):210–221. doi: 10.1111/j.1751-7915.2009.00136.x

Figure 3.

Figure 3

Flow cytometric analysis is used to estimate approximate genome size. Logarithmic YPD‐grown cells of the industrial (CR and CR20) and laboratory (BY4743) S. cerevisiae strains were stained with propidium iodine and the DNA content of 30 000 individuals cells was measured by flow citometry. The horizontal axes in the graphs are measures of dye fluorescence and are proxies for genome size. Diploid cells of the BY4743 strain were used as control. The first peak indicates the unreplicated DNA content of the population, G1 phase (2n for the BY4743 strain), while the second peak indicates the replicated DNA content, G2 phase (4n for the BY4743 strain). A representative experiment is shown.