Table 3.
Ethyl ester production in eht1Δ, eeb1Δ, ymr210wΔ (= ymrΔ) single and multiple deletion strains (Saerens et al., 2006).
Compound | wt | eht1Δ | eeb1Δ | ymrΔ | eht1Δ eeb1Δ | eht1Δ ymrΔ | eeb1Δ ymrΔ | eht1Δ eeb1Δ ymrΔ |
---|---|---|---|---|---|---|---|---|
Ethyl butanoate | 1.00 | 0.97 | 0.64 | 1.06 | 0.70 | 0.91 | 0.55 | 0.54 |
Ethyl hexanoate | 1.00 | 0.64 | 0.12 | 0.95 | 0.08 | 0.84 | 0.08 | 0.05 |
Ethyl octanoate | 1.00 | 0.80 | 0.55 | 0.89 | 0.28 | 0.85 | 0.24 | 0.10 |
Ethyl decanoate | 1.00 | 1.18 | 0.60 | 1.11 | 0.56 | 0.90 | 0.20 | 0.07 |
Gas chromatographic measurement of ethyl butanoate, ethyl hexanoate, ethyl octanoate and ethyl decanoate produced by the wild type (wt) and the deletion strains eht1Δ, eeb1Δ, ymr210wΔ, eht1Δ eeb1Δ, eht1Δ ymr210wΔ, eeb1Δ ymr210wΔ, and eht1Δ eeb1Δ ymr210wΔ after 96 h of fermentation. Standard deviations were typically 10% and did not exceed 20%. The level produced by the wt strain was set to 1.00 for each ester individually.