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. 2010 Feb 22;3(2):178–200. doi: 10.1111/j.1751-7915.2009.00122.x

Figure 1.

Figure 1

A. Sequence and unique restriction sites of pSILBAγ for inverted repeated sequence cloning. The acceptor and donor bases of L. bicolor nitrate reductase intron are in italics. The BamHI site in brackets is not unique. The same cloning sites are unique also in pSILBA and pSILBAα plasmid variants.
B. pSILBAγ cloning vector. SacI and NotI linearize the plasmid; XbaI liberates the silencing triggering cassette.
C. pSILBA.
D. pSILBAα.
Pgpd: glyceraldehide‐3‐phosphate dehydrogenase promoter of Agaricus bisporus. NR: nitrate reductase. CUT: cutinase. TtrpC: Aspergillus nidulans tryptophan synthetase terminador.