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. 2010 Feb 22;3(2):178–200. doi: 10.1111/j.1751-7915.2009.00122.x

Figure 2.

Figure 2

Two‐step cloning of the hairpin trigger in pSILBAγ and joining the plasmid with the binary vector pHg. The T‐DNA structures generated with pSILBAγ and pSILBAα are marked as pHg/pSγ and pHg/pSα respectively. SC: silencing cassette. HRC: hygromycin resistance cassette. MCS: multiple cloning site. LB: T‐DNA left border repeat of pCAMBIA1300. RB: T‐DNA right border repeat of pCAMBIA1300. hph: hph gene of E. coli coding for an aminocyclitol phosphotransferase that confers resistance to hygromycin B and structurally related antibiotics. Ampr: bla (ApR) gene of E. coli coding for a β‐lactamase that confers resistance to ampicillin. Kanr: aadA gene of E. coli coding for an aminoglycoside phosphotransferase that confers resistance to kanamycin. 35S‐3′: cauliflower mosaic virus 35S terminator.