Figure 5.

A. Laccaria wild‐type dikaryon and six pHg/pSILBAγ/NITRLoop‐transformed strains grown for 23 days on solid medium with ammonium or nitrate as N sources. Growth categories: N, non‐affected; A, affected; S, strongly affected.
B. Growth of wild type and transformants in liquid nitrate medium after 22 days.
C. Dry weight of mycelia produced by wild type and six pHg/pSILBAγ/NITRLoop‐transformed strains in liquid nitrate medium after 22 days.
D. Reverse transcription polymerase chain reaction (RT‐PCR) expression analysis of L. bicolor nitrate reductase‐encoding gene (protein ID 254066). Total RNA from mycelia of Laccaria S238N wild type and six pHg/pSILBAγ/NITRLoop‐transformed strains was isolated and used for first‐strand cDNA synthesis. A PCR was performed with first‐strand cDNA as template and between 25 and 30 cycles of amplification with alpha tubulin (control gene, protein ID 192524) and nitrate reductase primers. The picture shows fragments amplified after 30 cycles of PCR. For details see Experimental procedures.