Figure 7.

A. HpaII (CpG methylation sensitive)/MspI (CpG methylation insensitive) restriction map of the Laccaria nitrate reductase gene. The probes used for hybridization (1–3) and expected fragments from complete digestion of the DNA are represented above the gene with solid two‐headed arrows (37 bp under the detection level). The two smallest possible fragments originating from CpG‐methylated DNA digested with HpaII are represented by dashed two‐headed arrows. The 417 bp sequence used for triggering RNA silencing is presented below the gene.
B–D. CpG methylation analysis of the Laccaria nitrate reductase gene in wild type and in silenced strains. (B) NR probe 1. (C) NR probe 2. (D) NR probe 3. pSα refers to a strain transformed with pHg/pSILBAα/NITRLoop construct. pSγ refers to strain transformed with pHg/pSILBAγ/NITRLoop construct. H: HpaII restriction. M: MspI restriction. eg; main endogene signal. st: main silencing trigger signal.