Skip to main content
. 2010 Feb 22;3(2):178–200. doi: 10.1111/j.1751-7915.2009.00122.x

Figure 8.

Figure 8

A. Laccaria wild‐type monokaryon (wt), mutant with 5‐Pase gene interruption and 5‐Pase‐silenced strains.
B. Reverse transcription polymerase chain reaction (RT‐PCR) expression analysis of L. bicolor synaptojanin‐like 5‐Pase‐encoding gene (protein ID 306121). Total RNA from mycelia of Laccaria H82 wild type and two pHg/pSILBAγ/INOLoop transformants (I, II) was isolated and used for first‐strand cDNA synthesis. A PCR was performed with first‐strand cDNA as template and between 25 and 30 cycles of amplification with alpha tubulin (control gene, protein ID 192524) and 5‐Pase gene primers. The picture shows fragments amplified after 27 cycles. For details see Experimental procedures.