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. Author manuscript; available in PMC: 2013 Nov 21.
Published in final edited form as: Clin Cancer Res. 2012 May 1;18(9):10.1158/1078-0432.CCR-11-3167. doi: 10.1158/1078-0432.CCR-11-3167

Figure 5.

Figure 5

A novel mutation in the PIK3CA 3′ UTR affects the binding of miR-520a-5p and miR-525-5p. CRC007 (A) and CRC042 (B) had increased sensitivity to saracatinib. Data are presented as an average of treated (T)/control (C) × 100 at 28 days (end of study). A novel PIK3CA mutation in the 3′ UTR was identified in both explants by sequencing the PIK3CA gene in (A) CRC007 tumor and (B)CRC042 normal and tumor. C, Western blot analysis of the p110-α protein. Protein levels of the p110-α subunit were the greatest in CRC007. D, cotransfection of miR-520a or miR-525 mimic and wild-type psi-PIK3CA-T plasmid significantly decreases the luciferase activity from HEK293 cell lysates compared with control group. While cotransfection with the mutant identified in these studies psi-PIK3CA-C was not significantly different from the empty vector control. ***P < 0.001. CRC, colorectal cancer. CRC, colorectal cancer; WT, wild-type.