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. Author manuscript; available in PMC: 2013 Nov 21.
Published in final edited form as: J Neurochem. 2000 Jan;74(1):143–150. doi: 10.1046/j.1471-4159.2000.0740143.x

FIG. 3.

FIG. 3

RT-PCR shows S100β induction of IL-6 mRNA expression in neuronal cultures. A: Cortical neurons were incubated for 24 h either with S100β (100 ng/ml) that had been preabsorbed with anti-S100β antibody (lanes 2–4) or with S100β alone at the indicated doses. G3PDH mRNA was used to verify equal quantities of starting cDNA for each sample. B: Densitometric analysis of ethidium bromide-stained gels. IL-6 mRNA levels relative to G3PDH mRNA levels are expressed relative to values in control (Con) cultures. Data are mean ± SEM (bars) values for six to eight individual samples, **p < 0.01, significantly different from corresponding Con value. C and D: Competitive RT-PCR, performed on aliquots of samples used for analysis shown in lanes 2 and 8 of A. There is an ~10-fold difference in IL-6 mRNA expression between S100β-treated (D) and control (C) cultures.