Figure 2. Results of triplicate analyses of 10-fold serial dilutions of DNA for two assays.
(A) Amplification plot for the North American (NA)-specific SNP assay indicating cycle threshold values for 10-fold serial dilutions of DNA form isolate FV-1 (NA-specific allele). The average cycle threshold values for the alternate allele (isolate Nairobi) were similar (data not shown). (B) Amplification plot for the CO92-specific SNP assay indicating cycle threshold values for 10-fold serial dilutions of DNA from isolate CO92 (CO92-specific allele). The average cycle threshold values for the alternate allele (from isolate FV-1) were similar (data not shown).