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. 2013 Jul 25;5(3):392–410. doi: 10.3390/pharmaceutics5030392

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Immune response in mice vaccinated with 2.0 µg HA, free or mixed with Al(OH)₃ or with liposomes containing different immune modulators: HA-specific serum IgG1 after prime (A); and boost (B); IgG2a/c after boost (C); and HI titer after boost (D). For panels A–C, each dot represents the log serum titer of an individual mouse (non-responding mice were given an arbitrary titer of 10) and bars represent average log titer + SEM. For panel D, each dot represents the log HI titer in serum of an individual mouse and bars represent the geometric mean. Significant differences between the liposomal formulations and the DC-Chol:DPPC group are indicated with * (p < 0.05); significant differences between the liposomal formulations and the Al(OH)₃ group are indicated with ⁺⁺ (p < 0.01).