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. Author manuscript; available in PMC: 2014 Nov 15.
Published in final edited form as: J Immunol. 2013 Oct 14;191(10):10.4049/jimmunol.1302079. doi: 10.4049/jimmunol.1302079

Figure 7. KIR2DL4 associates with the heparan sulfate proteoglycan, SDC4 in HEK293T cells.

Figure 7

(A) HEK293T cells expressing KIR2DL4-mCherry and CD55-GFP (control) were complemented with standard culture medium (NT) or with culture medium containing 1μg/ml of either HS or CS. (B) HEK293T cells expressing KIR2DL4-mCherry and SDC4-GFP (experiment) were complemented with standard culture medium (non-treated; NT) or with culture medium containing 1μg/ml of either HS or CS. M2 coefficient corresponds to the proportion of KIR2DL4-mCherry co-localized with the appropriate GFP-labeled marker. Data represent mean ± s.d. of indicated number of biological replicas (N) for n=3 independent experiments. P-values were calculated using T-test. T-test (control): N=37; *p-value and **p-value ≥ 0.9 (no difference); T-test (experiment): N=55; *p-value and **p-value ≤ 0.005. (C) Representative images of non-treated (NT) and HS or CS treated cells are shown.