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. Author manuscript; available in PMC: 2014 Sep 27.
Published in final edited form as: Science. 2013 Aug 22;341(6153):10.1126/science.1240636. doi: 10.1126/science.1240636

Fig. 3. BMAL1 regulates rhythmic oscillations of Ly6Chi monocytes in a cell autonomous manner.

Fig. 3

(A) Quantitative RT-PCR kinetic analysis of Nr1d1 mRNA in blood monocytes isolated from ArntlLoxP/LoxP and ArntlLoxP/LoxPLyz2Cre mice kept on a 12 hour light-dark cycle (n = 3-4 samples per genotype and time point). (B to D) Ly6Chi monocyte numbers in blood (B), spleen (C), and bone marrow (D) of ArntlLoxP/LoxP and ArntlLoxP/LoxPLyz2Cre mice kept under a 12 hour light-dark cycle at various ZTs. (n = 5 mice per genotype and time point). (E) Survival curves of ArntlLoxP/LoxP and ArntlLoxP/LoxPLyz2Cre mice after infection with 1×106 L. monocytogenes at ZT0 and ZT8 (n = mice 10-11 per genotype and time point). (F to I) Serum concentrations of IL1β (F), IL6 (G), IFNγ (H), and CCL2 (I) in ArntlLoxP/LoxP and ArntlLoxP/LoxPLyz2Cre mice 2 dpi with 1×106 L. monocytogenes at ZT0 and ZT8 (n = mice 4-6 per genotype and time point). Pooled data (A and E) and representative (B to D) of two to three independent experiments are shown as mean ± SEM and analyzed using two-tailed Student's t-tests (A to D, and F to I), and log-rank test (E). *P<0.05; **P<0.01; ***P<0.001.