Table 2. Primer sets for the reverse transcription and real-time RT-qPCR.

Target	Purpose	Primer Name	Sequence (5′-3′)	Position (nt)
mRNA	reverse transcription	Oligo tagdTRT rev	GTAAAACGACGGCCAGTTTTTTTTTTTTTTTTT	polyA tail
	real-time RT-qPCR	Seg 5 Realtime for	GGAAAGTGCAAGACCAGAAGAT	1388–1410
	real-time RT-qPCR	mRNA tagRealtime rev	GTAAAACGACGGCCAGT	tag sequence
cRNA	reverse transcription	Seg 5 tagRT rev	GCTAGCTTCAGCTAGGCATCAGTAGAAACAAGGGTATTTTTCTT	1541–1565
	real-time RT-qPCR	Seg 5 Realtime for	GGAAAGTGCAAGACCAGAAGAT	1388–1410
	real-time RT-qPCR	cRNA tagRealtime rev	GCTAGCTTCAGCTAGGCATC	tag sequence
vRNA	reverse transcription	Seg 5 tagRT for	ATTTAGGTGACACTATAGAAGCGAGTGATTATGAGGGACGGTTGAT	192–215
	real-time RT-qPCR	Seg 5 Realtime rev	CGCACTGGGATGTTCTTC	282–300
	real-time RT-qPCR	vRNA tagRealtime for	ATTTAGGTGACACTATAGAAGCG	tag sequence