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. 2013 Nov 21;9(11):e1003904. doi: 10.1371/journal.pgen.1003904

Figure 4. Functional analysis of Pax6_ciCNE2 deletion constructs.

Figure 4

(A) Schematic representation and quantification of the constructs injected in the zebrafish embryos to examine their enhancer activity. The numbers in parentheses indicate the length of each construct. (B) View of a 48 hpf embryo injected with the full length CNE2 expressing GFP in sensory neurons (arrow). In the embryos injected with DCNE2-1 (C) or DCNE2-2 (D) GFP is detected in sensory neurons (arrow). The minimal construct DCNE2-1-2+DCNE2-2-1 (E) is able to drive GFP expression in neurons along the spinal cord (arrow).