Table 1. Summary of GFP reporter expression driven by ciCNEs at 48Tol2 and co-injection strategies in zebrafish embryos.
| Tol2 strategy | Co-injection strategy | ||||||
| Human gene name | Ciona Ensembl gene | # of Ciona CNEs | CNE Name | # of embryos (injections) | % embryo with phenotype | expression profile | |
| PAX6 | ENSCING00000006503 | 2 | Pax6_ciCNE1 | 256 (2) | Negative | ||
| Pax6_ciCNE2 | 195 (3) | 12 | spinal cord sensory neurons and pre-otic ganglia neurons | 4/142 (neurons) (2.8%) | |||
| OTX1/OTX2 | ENSCING00000008859 | 1 | Otx_ciCNE1 | 150 (3) | Negative | ||
| NKX2-4 | ENSCING00000006231 | 3 | Nkx_ciCNE1 | 146 (2) | 4 | sensory pre-otic ganglion | |
| Nkx_ciCNE2 | 150 (2) | Negative | |||||
| Nkx_ciCNE3 | 110 (2) | Negative | |||||
| OTP | ENSCING00000004120 | 1 | Otp_ciCNE1 | 112 (2) | Negative | ||
| PHOX2B-like | ENSCING00000009645 | 1 | PhoxB_ciCNE1 | 120 (2) | Negative | ||
| MEIS1/MEIS2 | ENSCING00000005356 | 10 | Meis_ciCNE1 | 180 (2) | 50 | motor neurons and interneurons | 40/184 (neurons) (22%) |
| Meis_ciCNE2 | 160 (2) | 5 | sensory post-otic ganglion | ||||
| Meis_ciCNE3 | 116 (1) | Negative | |||||
| Meis_ciCNE4 | 240 (3) | 6 | sensory post-otic ganglion | ||||
| Meis_ciCNE5 | 160 (2) | Negative | |||||
| Meis_ciCNE6 | 360 (3) | Negative | |||||
| Meis_ciCNE7 | 168 (2) | Negative | |||||
| Meis_ciCNE8 | 315 (3) | 4 | mixed population of sensory and interneurons | ||||
| Meis_ciCNE9 | 236 (3) | Negative | |||||
| Meis_ciCNE10 | 285 (3) | 20 | spinal cord sensory neurons (Rohon-beard) and trigeminal ganglion neurons | Less than 1% (neurons) | |||
| EBF3 | ENSCING00000003209 | 1 | Ebf3_ciCNE1 | 70 (1) | Negative | ||
| ZFHX1B | ENSCING00000006930 | 2 | Zfhx_ciCNE1 | 180 (2) | Negative | ||
| Zfhx_ciCNE2 | No PCR | N/A | |||||
| HHEX | ENSCING00000001270 | 1 | Hhex_ciCNE1 | 321 (3) | 12 | macrophage-like | 20/82 (24%) |