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. 2013 Nov 21;9(11):e1003971. doi: 10.1371/journal.pgen.1003971

Figure 2. Molecular confirmation of recombination in WT, but not xrcc2 mutant plants.

Figure 2

Schematic representation of the GU.US recombination tester locus (A) and Southern analysis (B) of DNA from plants grown in the absence (lanes 1 to 6) or presence of β-estradiol (lanes 7 to 10), digested with SacI (lanes 1,3,4,7,8) or SacI plus I-SceI (lanes 2,5,6,9,10). The blot was hybridized with a GUS-specific probe as indicated in panel (A). The recombined GUS gene has lost its I-SceI site and is seen as a single 2.5 kb SacI fragment only in DNA from WT plants grown in presence of β-estradiol (lanes 7 and 9). Col0: WT plants of Columbia ecotype.