Skip to main content
. 2013 Nov 21;9(11):e1003773. doi: 10.1371/journal.ppat.1003773

Figure 5. Type I and III are redundant in the MTEC system.

Figure 5

(A) Total RNA from mock and PR8 infected cells was analysed using Affymetrix Mouse Genome 430 2.0 microarrays at 24 hpi. Supervised analysis was performed using statistical filtering (≥4-fold change relative to mock infected wild-type in at least one treatment group; 2-way ANOVA, p<0.01, Benjamini-Hochberg multiple test correction). (A) Heat map of the upregulated genes. (B) Quantitative RT-PCR analysis of RNA samples extracted from mock and PR8 infected wild-type and IFNAR1−/−IL-28Rα−/− double knock-out MTEC at 24 and 48 hpi. All transcripts were first normalized to HPRT levels and then expressed as fold induction relative to the mean of mock infected controls, +/− SEM. (C) Wild-type and IFNAR1−/−IL-28Rα−/− double knock-out MTEC were infected and RNA samples collected at the indicated time points. Viral replication was measured by qRT-PCR on the Influenza A Matrix gene and expressed as copy number +/− SEM. Asterisks indicate differences that are statistically significant (unpaired t test; *, P<0.05).