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. 2013 Nov 21;8(11):e81105. doi: 10.1371/journal.pone.0081105

Figure 1. Western blots for neural stem cell and neuroprogenitor cell proteins in piglet forebrain.

Figure 1

A. Nestin immunoreactivity in forebrain subventricular zone (SVZ) and olfactory bulb (OB) SVZ. Lower four blots show immunoreactivities for Dlx2 (B), musashi (C), doublecortin (D), and PSA-NCAM (E) in OB SVZ subcellular extracts (membrane, soluble and crude nuclear) at postnatal day (PND) 1 and PND 30. Molecular weight standards (in kDa) are shown at right side of the blots. F. Representative Ponceau S-stained nitrocellulose membrane is shown for protein loading. G. Graph showing the densitometry quantification of the levels of immunoreactivity for Dlx-2, musashi, doublecortin, and PSa-NCAM in membrane (Dlx-2 and PSA-NCAM) and soluble (musashi and doublecortin) fractions in the OB periventricular region at PND1 and PND30. Values are mean ± SD. Single asterisk denotes musashi PND30 vs PND1 p < 0.05. Double asterisk denotes PSA-NCAM PND1 vs PND30 p < 0.001.