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. 2013 Nov 21;8(11):e81105. doi: 10.1371/journal.pone.0081105

Figure 3. Localization of NSC and NPC markers in piglet forebrain SVZ and white matter.

Figure 3

A and B. Immunohistochemical localization of nestin (A) and musashi (B) shows enrichment of these two NSC markers in piglet forebrain SVZ. Immunoreactivity is seen as orange-brown staining. Arrows identify the SVZ. The lateral ventricle is seen at right. The opaque layer at the interface of the SVZ and the ventricular cavity is the ciliated ependymal layer. Scale bar = 33 µm. C and D. Immunohistochemical localization of nestin (C) and Dlx2 (D), a neuroprogenitor marker, shows enrichment in individual cells (arrows) in piglet subcortical white matter. Scale bar in C (same for D-F) = 17 µm. E and F. Nestin primary antibody (E) was preadsorbed against nestin synthetic peptide, and Dlx2 primary antibody (F) was preadsorbed against recombinant Dlx2. No staining is seen in either preparation. G. Graph showing the number of nestin+ and Dlx2+ cell bodies in the subcortical white matter of the parasagittal gyrus in primary somatosensory cortex in piglets at postnatal day (PND) 5 and 30. Values are mean ± SD (n= 3-4 piglets/group). Asterisk denotes significant difference (p < 0.05) compared to PND5.