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A. Floating neurosphere formed by notch-1-immunopanned NSCs isolated from the OB. Scale = 12 µm. B-D. Attached neurospheres dispersed as monolayers are multipotent by differentiating into cells that are neurons identified by MAP2, astrocytes identified by GFAP, and oligodendrocytes identified by O4. E. Neurosphere density increased when medium was supplemented with FGF2. Scale bars = 12.5 (B), 20 (C), 20 (D), 50 (E) µm.
