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. 2013 Nov 21;8(11):e80723. doi: 10.1371/journal.pone.0080723

Figure 6. Comparison of RSV infection in Ifitm3-/- mice to wild type mice.

Figure 6

Ifitm3-/- or wild type littermate control mice were infected i.n. with 5x105 RSV A2. Mice were weighed daily and weight changes recorded as a percentage of original weight (A). Lungs were excised and viral load calculated by qPCR on days 4 and 7 pi (B). Total cell counts from lung (C) and BAL (D) were calculated, along with totals of CD3, CD4 and CD8 (T cells), CD19 (B cells) and DX5+ (NK cells) (E) measured in lung by flow cytometry on day 7 post infection. Granulocyte numbers were also calculated in the BAL on day 7 post infection (F) Levels of the inflammatory cytokines IFNγ and IL-1β in lung (G) and IFNγ and IL-6 in BAL (H) were measured by ELISA on day 7 post infection. ■: wild type, □: Ifitm3-/-. Results show means ± S.D. (n > 5). Statistical significance was assessed by Student’s t-test, or ANOVA followed by Bonferroni's Multiple Comparison Test when there were more than two groups (* p<0.05, ** p<0.01, *** p<0.001).