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. 2013 Nov 21;8(11):e81525. doi: 10.1371/journal.pone.0081525

Figure 3. Melting peaks of the SYBR Green Ⅰ-based one-step qRT-PCR products.

Figure 3

(A) Melting peaks of the qRT-PCR products obtained from in vitro transcribed HTNV cRNA ranging from 1×108 to 1×103 copies/µl. Only a single sharp peak at 84°C was visible in the melt peak chart. (B) Melting peaks obtained from serum samples of HFRS patients. All melting curves in A and B have similar shapes.