Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
(A) Serial dilutions of the HTNV cRNA standards ranging from 1×106 to 1×10-1 copies/µl were amplified using the SYBR Green Ⅰ-based one-step qRT-PCR assay in duplicate, and the minimum detection limit of the qRT-PCR assay was 10 copies/µl. (B) The qRT-PCR products were electrophoresed on a 2% agarose gel. Marker: DNA ladder of DL2000.
