Table 2. Quantification of HTNV RNA in serum samples from patients and normal controls using the SYBR Green I-based one-step qRT-PCR assay.
| Sample Number | HTNV-IgM/IgG Antibodies Combo test | Days after onset of fever | Severity of the HFRS disease * | Copies/µl of the HTNV RNA |
|---|---|---|---|---|
| HFRS 1 | Positive/Positive | 4 | Critical | 8.64×102 |
| HFRS 2 | Positive/Positive | 5 | Critical | 1.43×103 |
| HFRS 3 | Positive/Positive | 5 | Severe | 2.86×103 |
| HFRS 4 | Positive/Positive | 4 | Moderate | 4.23×102 |
| HFRS 5 | Positive/Negative | 6 | Severe | 1.49×104 |
| HFRS 6 | Positive/Positive | 3 | Mild | 1.47×104 |
| HFRS 7 | Positive/Positive | 6 | Critical | 1.83×104 |
| HFRS 8 | Positive/Positive | 6 | Mild | 1.25×103 |
| HFRS 9 | Positive/Positive | 4 | Mild | 5.52×102 |
| HFRS 10 | Positive/Positive | 2 | Mild | 1.05×105 |
| HFRS 11 | Positive/Positive | 4 | Severe | 1.95×105 |
| HFRS 12 | Positive/Negative | 6 | Moderate | 7.44×102 |
| HFRS 13 | Positive/Negative | 4 | Moderate | 1.78×103 |
| HFRS 14 | Positive/Positive | 4 | Critical | 1.23×104 |
| HFRS 15 | Positive/Positive | 4 | Severe | 1.06×104 |
| HFRS 16 | Positive/Positive | 4 | Severe | 3.64×104 |
| HFRS 17 | Positive/Positive | 5 | Moderate | 1.13×104 |
| HFRS 18 | Positive/Positive | 5 | Mild | 2.06×101 |
| HFRS 19 | Positive/Positive | 3 | Moderate | 3.26×101 |
| HFRS 20 | Positive/Positive | 3 | Moderate | 3.32×104 |
| HFRS 21 | Positive/Negative | 3 | Mild | 4.37×103 |
| HFRS 22 | Positive/Positive | 5 | Moderate | 8.39×103 |
| HFRS 23 | Positive/Positive | 3 | Severe | 1.62×102 |
| HFRS 24 | Positive/Positive | 4 | Moderate | 7.99×102 |
| HFRS 25 | Positive/Negative | 5 | Mild | 5.04×102 |
| HFRS 26 | Positive/Positive | 3 | Mild | 1.03×102 |
| HFRS 27 | Positive/Negative | 3 | Moderate | 1.63×102 |
| HBV 1 | Negative/Negative | - | - | Negative |
| HBV 2 | Negative/Negative | - | - | Negative |
| HBV 3 | Negative/Negative | - | - | Negative |
| HCV 1 | Negative/Negative | - | - | Negative |
| HCV 2 | Negative/Negative | - | - | Negative |
| HCV 3 | Negative/Negative | - | - | Negative |
| HIV 1 | Negative/Negative | - | - | Negative |
| HIV 2 | Negative/Negative | - | - | Negative |
| HIV 3 | Negative/Negative | - | - | Negative |
| NC 1 | Negative/Negative | - | - | Negative |
| NC 2 | Negative/Negative | - | - | Negative |
| NC 3 | Negative/Negative | - | - | Negative |
| NC 4 | Negative/Negative | - | - | Negative |
*
, The severity of HFRS disease was classified on the basis of clinical manifestations and laboratory parameters used in the diagnostic criteria for HFRS in China as mild, moderate, severe, and critical. NC, Normal control.