Figure 2. DAG-based lipoglycans are recognized by TLR2 in cooperation with TLR1 or TLR6.

A, C) HEK-TLR2 cells were incubated with the various stimuli at a concentration ranging from 0.1 to 1000 ng/ml. B) HEK-TLR2 cells were pre-incubated or not for 30 min at 37°C before stimuli addition with various monoclonal antibodies: anti-TLR2 (5 µg/ml), anti-TLR1 (10 µg/ml), anti-TLR6 (10 µg/ml) or IgG1 isotype control (10 µg/ml). FSL-1 was tested at a concentration of 1 ng/ml, Pam₃CSK₄ and MsLM at 10 ng/ml, SmLM at 50 ng/ml, MlLM and SaLTA at 100 ng/ml and CgLM at 300 ng/ml. In C, deacylated molecules (+) were prepared by treating native molecules (−) with 2M NH₄OH for 2h at 110°C. NF-κB activation was determined by reading OD at 630 nm. The results are mean ± SD of triplicate wells and are representative of at least three separate experiments. ***, P<0.001. Iso ctrl, isotype control, n.i., not induced.